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KMID : 1237720110440020098
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Anatomy & Cell Biology
2011 Volume.44 No. 2 p.98 ~ p.105
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Gene expression profiling of mouse aborted uterus induced by lipopolysac charide
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Moon Jeong-Mi
Lee Song-Eun
Min Yong-Il
Jung Chae-Yong
Ahn Kyu-Youn
Nam Kwang-Il
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Abstract
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To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4,Ccl2,Cxcl13,Gbp3,Gbp2,Mx2,H2-Eb1,Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. The results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
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KEYWORD
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Abortion, Lipopolysaccharide, Microarray, Mouse
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